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    Widal Test (Slide Method)44635022% off
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    What is a Widal Test (Slide Method) ?

    The Widal test is a blood test used to measure antibody titres when an individual is suspected to have typhoid or paratyphoid fever (collectively known as enteric fever). Antibody titres measure the presence and amount of these specific antibodies in the blood, which in turn reflects the body's immune response when it is exposed to antigens (foreign substances). The Widal test specifically detects antibodies against the O and H antigens of Salmonella Typhi and H antigens of Salmonella Paratyphi . Introduced by F. Widal in 1896, this blood test uses a suspension medium containing killed Salmonella Typhi bacterium as the antigen to identify the presence of typhoid fever in patients presenting with fever. Therefore, anyone exhibiting symptoms and indications of enteric fever may benefit from a Widal test.

    Given its role in diagnosing enteric fever, the Widal test can be a critical diagnostic tool aiding in timely detection and treatment. Therefore, if you live in Bangalore and are experiencing symptoms of enteric fever, consider getting a Widal test in Bangalore today.

    Widal Test (Slide Method) Parameters

    The Widal test is an agglutination test, which means it detects the clumping of cells caused when particular antibodies (agglutinins) in the blood react to specific antigens. These antibodies target two key antigens of Salmonella Typhi : the O antigen and the H antigen. Additionally, the test includes the H antigens of Salmonella Paratyphi . Thus, the detection of these antibodies helps in diagnosing enteric fever by indicating the body's immune response to these bacterial infections.

    The Widal test has four parameters, which represent the levels of antibody titres against the bacteria responsible for enteric fever. The parameters of a Widal test include:

    • Salmonella Typhi O (TO)

    • Salmonella Typhi H (TH)

    • Salmonella Paratyphi A, H (AH)

    • Salmonella Paratyphi B, H (BH)

    Widal Test (Slide Method) Preparation

    Before booking the Widal test in Bangalore, consider the following factors:

    Prerequisites

    Fasting is not necessary for the Widal test.

    Best Time to Get Tested

    The Widal Test can be scheduled at any time during the day.

    Who Should Get a Widal Test

    Individuals with symptoms such as fever, fatigue, headache, and gastrointestinal issues (abdominal pain, constipation, or diarrhoea), especially if they have been in areas with a high risk of typhoid or paratyphoid fever, should get a Widal test.

    Cautions Before Taking the Widal Test

    There are no special precautions for this test.

    Why Take a Widal Test (Slide Method) ?

    The Typhoid bacteria typically have an incubation period of 6 to 14 days, although it can be as long as 30 days. The incubation period is the interval of time between the infection with the bacteria and the onset of symptoms. For the paratyphoid bacteria, it might be anything from 1 to 10 days. Most of the time, symptoms gradually worsen over the course of 3 to 4 days.

    A doctor might request a Widal test if you have symptoms of typhoid fever. Similar, but less severe symptoms are also seen with paratyphoid fever. 6 These signs and symptoms include:

    • High fever or chills

    • Headache

    • Extreme fatigue

    • Feeling unwell, weak or sick

    • Pain in the abdomen

    • Constipation

    • Diarrhoea

    • Sweating

    • Blood in the stool

    • Sore throat and persistent cough

    • Bleeding from the nose

    • Weight loss

    • Decreased heart rate

    • Loss of appetite

    • Red rash on the chest and abdomen

    • Confusion or hallucinations

    • Difficulty in concentrating

    • Poisoning of blood

    • Enlarged spleen or liver

    Even those vaccinated against typhoid are not entirely immune, as 20–50% of vaccinated individuals may still contract the disease upon exposure.

    Individuals with an increased risk of typhoid may also be recommended a Widal test. These include:

    • People who are in close proximity to an infected individual.

    • Young children, as they are more vulnerable than adults to these diseases.

    • Travellers to regions where typhoid and paratyphoid are prevalent (such as India, Bangladesh, Middle Eastern countries, and African countries.)

    Widal Test (Slide Method) Results & Interpretation

    Below is a breakdown of the results of the Widal test:

    TypeAntibodyTitreInterpretation
    Salmonella TyphiTO<1:80Negative
    Salmonella TyphiTH<1:80Negative
    Salmonella ParatyphiAH<1:80Negative
    Salmonella ParatyphiBH<1:80Negative

    Interpretation

    A titre less than 1:80 is considered negative, indicating the patient does not have an infection.

    Titres of 1:80 or higher are typically considered positive, although this threshold may vary among different populations and regions. A rising titre, observed through two or more samples taken at 4- to 6-day intervals after the onset of the disease, is considered more significant than a single high titre. The results of the test should be correlated with the patient’s medical findings to reach a final diagnosis.

    Limitations

    Patients vaccinated against typhoid may display elevated antibody titres, potentially leading to false-positive results. Similarly, the results may be misleading in individuals with prior infections or immunisations.

    Agglutinins in blood generally appear by the end of the first week of infection, so blood samples taken earlier may yield negative results.

    Although the serological findings of this test are valuable, they should not replace culture-based methods for identifying the causative organisms. Efforts should be made to recover and identify the pathogens through culture and biochemical tests.

    FAQs on Widal Test (Slide Method) in Bangalore

    The Widal price is ₹ 350. This price includes the fastest home sample collection within 60 minutes of booking, and the reports are available in 6 hours.

    A Widal test or blood test for typhoid is used to detect the presence of antibodies in the blood against specific antigens of Salmonella bacteria, particularly Salmonella Typhi and Salmonella Paratyphi . It aids in diagnosing enteric fever, including typhoid and paratyphoid fever, by indicating the body's immune response to these bacterial infections.

    No, fasting is not needed for the Widal test or Typhoid Test at home.

    Yes, with Orange Health Labs, you can schedule home sample collection for the Widal Blood test or Typhoid Blood test with the convenience of sample collection within 60 of booking (based on slot availability).

    Listed below are the steps to book any blood test or health checkup on our platform

    • Search for the test: Look for the Ferritin Test in Bangalore or at home and click on Orange Health’s listing.

    • Review and Book: Select the test, check the test prerequisites, enter your address, and confirm the booking after picking a suitable time slot for sample collection.

    • Sample Collection: Our skilled eMedic will arrive within your chosen time slot to collect the sample at your home.

    • Lab Processing: The collected sample will be sent to and examined at our NABL accredited and ICMR approved labs.

    • Receive Results: You will receive your reports via email or WhatsApp within 6 hours. They will also be available on our app.

    There isn't a standard ‘normal’ range for the Widal test. Instead, titres below 1:80 are typically considered negative, while titres of 1:80 or higher may suggest infection. However, interpretation depends on clinical factors and the prevalence of the disease in the region.

    After sample collection, you will receive your results within 6 hours via WhatsApp and the app if you’re in Bangalore. The time may vary in other cities.

    The frequency of Widal testing depends on your doctor's suggestion. If you've been diagnosed with typhoid or paratyphoid fever, your doctor may advise undergoing this test every 2 weeks, tailored to your clinical condition and symptoms.

    The parameters included in the Widal test are the titres of antibodies against specific antigens of Salmonella bacteria, such as the O and H antigens of Salmonella Typhi and H antigens of Salmonella Paratyphi .

    The Widal test indicates the presence of antibodies against Salmonella bacteria, aiding in the diagnosis of typhoid and paratyphoid fever.

    The Slide method involves mixing serum and antigen on a slide, while the Tube method mixes them in test tubes.

    A blood test carries minimal danger. You may have some discomfort at the site of the needle puncture during sample collection, but it is short-lived.

    A false-negative Widal test wrongly suggests no antibodies when they're present. This may happen if the test is taken too early. A false-positive result wrongly suggests antibodies. This may be possible due to past infections or vaccinations.

    Without having to visit a physical lab, Orange Health provides the fastest Widal test services near you across Bangalore at the convenience of your home. Localities include but not limited to Adugodi, Banashankari, Bannerghatta, Basavanagudi, Bellandur, BTM Layout, Chamrajpet, Cottonpet, CV Raman Nagar, Domlur, Electronic City, Frazer Town, Giri Nagar, Hebbal, Hennur, Hosur, HSR Layout, Jakkur, Jalahalli, Jaya Nagar, Kadugodi, Kammanahalli, Kengeri, Koramangala, Mahalakshmi Layout, Madiwala, Magadi Road, Malleshwaram, Marathahalli, Mathikere, Mysore Road, Nagarbhavi, Nandhini Layout, Old Airport Road, Peenya, RT Nagar, Rajaji Nagar, Rajarajeshwari Nagar, Sadashivnagar, Sahakara Nagar, Sanjay Nagar, Sarjapur, Seshadripuram, Shanthi Nagar, Shivaji Nagar, Sri Nagar, Tyagarajnagar, Uttarahalli, Vasanth Nagar, Vidyaranyapura, Vijaya Nagar, Whitefield, Yelahanka, Yeshwantpur.

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