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    What Is the HLA-B27, Qualitative PCR Test?

    The HLA B27 Qualitative PCR Test is a blood test that helps detect the presence of the HLA-B27 protein on white blood cells, which is an important part of the immune system. This protein helps the body fight infections, but its presence in some individuals can also increase the risk of autoimmune diseases, where the immune system attacks the body's healthy cells.

    Conditions frequently associated with HLA-B27 include:

    • Juvenile arthritis : A type of inflammatory arthritis that affects children.

    • Ankylosing spondylitis : A condition that primarily affects the spine, leading to pain and stiffness.

    • Reactive arthritis : Joint inflammation caused by an infection.

    A positive HLA-B27 test result indicates the protein is present in the blood, which may increase the chance of developing certain autoimmune conditions. However, it does not serve as a definitive diagnosis. In fact, many individuals with the HLA-B27 gene never develop these diseases. Additional testing—such as X-rays, MRIs, urine tests, and measurements of C-reactive protein (CRP) and Erythrocyte Sedimentation Rate (ESR)—may be necessary for a more accurate diagnosis.

    If the test result is negative, this means HLA-B27 is absent, significantly lowering the likelihood of developing these autoimmune diseases. For those needing organ or tissue transplants, mismatched HLA types can increase the risk of transplant rejection.

    If you are residing in Noida, getting the HLA B27 Qualitative PCR test at home is convenient with facilities like free home sample collection and prompt results.

    HLA-B27, Qualitative PCR Test Parameters

    The HLA B27 Qualitative PCR Test is designed to identify the presence of the HLA-B27 antigen on white blood cells. This antigen is part of the broader family of human leukocyte antigens (HLA), which are essential in helping the immune system distinguish between the body’s own cells and harmful invaders like viruses and bacteria.

    HLA proteins are divided into two main categories: Class I (A, B, C) and Class II (DR, DP, DQ). Although not as distinctive as fingerprints, these proteins show variability from person to person and play a crucial role in the body’s immune response. The HLA-B27 antigen's presence is determined by genetics and is inherited from parents.

    There are around 15 different subtypes of HLA-B27, with the most common being HLA-B2705 and HLA-B2702. Some subtypes may increase the likelihood of developing certain autoimmune diseases. While ongoing research is exploring how different subtypes affect the risk of disease, having HLA-B27 doesn’t necessarily mean that a person will experience health issues. However, its presence can be a helpful factor in diagnosing specific autoimmune conditions.

    A person with a family history of HLA-B27-related diseases, such as ankylosing spondylitis, may have a higher risk of developing a similar condition. It is important to remember that genetics plays a key role, and while the presence of the antigen is informative, it does not guarantee the development of a disease.

    HLA-B27, Qualitative PCR Test Preparation

    You should be aware of the following things if you are planning to take the HLA B27 Qualitative PCR test at home:

    • Prerequisites : No special preparations are required before the test, like there is no need for fasting.

    • Best Time to Get Tested : This test can be done at any time of the day suitable to you.

    • Who Should Get a HLA B27 Qualitative PCR Test? : The test is advised for individuals with recurring eye inflammation (uveitis), autoimmune conditions, or persistent joint pain, swelling, or stiffness. The HLA B27 Qualitative PCR test is also crucial for ensuring compatibility in organ or tissue transplant cases.

    • Cautions Before Taking a HLA B27 Qualitative PCR Test : No specific precautions are required for the HLA B27 Qualitative PCR test.

    Why Take the HLA-B27, Qualitative PCR Test?

    The HLA-B27 test may be advised in a variety of situations where inflammatory or autoimmune conditions are suspected. Reasons for recommending the test include:

    • Persistent pain, swelling, or stiffness in the joints, which could point to an autoimmune disorder.

    • Chronic inflammation affecting areas like the eyes, joints, neck, chest, or spine, raising the possibility of ankylosing spondylitis or reactive arthritis.

    • Recurring uveitis without a clear cause, which might indicate an autoimmune issue.

    Other reasons for the test include:

    • Screening for the likelihood of developing HLA-B27-related autoimmune conditions like ankylosing spondylitis.

    • Assessing compatibility between donor and recipient prior to an organ or bone marrow transplant.

    The HLA B27 Qualitative PCR test is typically supplemented by other tests, including CRP, ESR, CCP antibody, rheumatoid factor, and X-rays to evaluate inflammation, joint damage, and autoimmune disorders.

    FAQs on HLA-B27, Qualitative PCR Test in Noida

    The HLA B27 Qualitative PCR test in Noida can be done for ₹2500, the price including home sample collection that is done within 60 minutes of booking. The test results are promptly delivered within 82 hours after sample collection with Orange Health Labs.

    Yes, Orange Health Labs provides free home sample collection facility for the HLA B27 Qualitative PCR test. A trained eMedic professional will arrive at your location within 60 minutes once your booking is confirmed, to collect the sample conveniently.

    The HLA B27 Qualitative PCR test is used to assess for the presence of the HLA-B27 antigen, which is linked to autoimmune disorders like, reactive arthritis, psoriatic arthritis and ankylosing spondylitis. It also assists in determining compatibility for organ transplant.

    Fasting is not required for the HLA B27 Qualitative PCR test. This test can be done at any time of the day as per your convenience.

    Booking your HLA B27 Qualitative PCR test with Orange Health Labs is straightforward. Just follow these steps:

    • Find the Test: Search for the "HLA B27 Qualitative PCR test" and select Orange Health Labs from the results.

    • Confirm Your Appointment: Enter your personal details, select your location, and choose a time for the sample collection that works for you.

    • Collection of Sample: A skilled eMedic professional will come to your home to take the blood sample.

    • Testing & Results: Your sample will be analyzed at a NABL-accredited, ICMR-approved lab. Expect to receive your results via email or WhatsApp within 82 hours after the sample collection.

    The HLA-B27 Qualitative PCR test shows a positive or negative result. A positive result suggests an increased risk for autoimmune diseases but doesn't confirm them, and a negative result is normal. 1,3

    The HLA B27 Qualitative PCR test results are usually available within 82 hours after sample collection.

    The frequency of this test depends on your health condition, symptoms, and your doctor's advice.

    The HLA B27 Qualitative PCR test checks for a single parameter: the presence of HLA-B27 antigen in the blood.

    The HLA B27 Qualitative PCR test is available in multiple locations across Noida, including Sector 1, Sector 15, Sector 18, Sector 26, Sector 37, Sector 50, Sector 62, Sector 76, Sector 93, Sector 100, Sector 104, Sector 110, Sector 120, Sector 128, Sector 137, Sector 150, Sector 168, Sector 71, Sector 63, Sector 22, Sector 41, Sector 108, Sector 47, Sector 121, Sector 45.

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    References

    1. HLA-B27 antigen: MedlinePlus Medical Encyclopedia.  Available from:  https://medlineplus.gov/ency/article/003551.htm
    2. HLA-B27 Antigen.  Available from:  https://www.urmc.rochester.edu/encyclopedia/content?ContentTypeID=167&ContentID=hla_b27_antigen
    3. Human leukocyte antigen (HLA) B27 | Pathology Tests Explained.  Available from:  https://www.pathologytestsexplained.org.au/ptests-pro.php?q=HLA+B27