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    What is a Dengue NS1 Antigen ELISA Test?

    The Dengue NS1 Antigen ELISA test detects the non-structural protein 1 (NS1) of the dengue virus in blood. It targets specific antigens, which are foreign substances entering the body. This detection is possible in the acute phase, or within the initial 7 days of dengue fever, aiding in early diagnosis and timely medical care.

    Dengue fever is spread by mosquito bites and is caused by one of the four serotypes of the dengue virus from the Flavivirus genus. When an infected mosquito bites a person, the virus enters the bloodstream and begins replication. This leads to the symptoms of dengue fever which are caused by the virus and the immune system’s response. The Dengue NS1 test can identify the virus in blood within the first 7 days of symptoms. Studies reveal that NS1 antigen levels in the blood correlate with the severity of the disease, with higher levels present in more severe infections. Hence, the Dengue Antigen test provides essential diagnostic information for dengue infection.

    For Hyderabad residents experiencing dengue symptoms, a Dengue Antigen test is highly beneficial. Arrange your Dengue Antigen test in Hyderabad today if you suspect dengue exposure to confirm your status.

    Dengue NS1 Antigen ELISA Test Parameters

    Dengue spreads through the bite of Aedes mosquitoes. When a mosquito bites a dengue-infected person and then bites a healthy one, it transmits the virus. The dengue virus has the ability to destroy blood components that are crucial for clotting and maintaining blood vessel integrity. This damage, combined with chemicals from the immune system, can lead to blood leaking from vessels, causing internal bleeding. Therefore, timely testing is important if symptoms appear.

    The Dengue NS1 Antigen test has one key parameter: it focuses on detecting the dengue NS1 protein, released into the blood during infection. This test uses a blood sample and special antibodies to identify the presence of the NS1 antigen. Antibodies are immune system proteins that respond to antigens like NS1. Detection of NS1 in the blood indicates an acute dengue virus infection, though its absence does not rule out infection. Doctors may order additional tests to confirm your diagnosis.

    Dengue NS1 Antigen ELISA Test Preparation

    Here is how to prepare for a Dengue Fever NS1 Antigen test:


    Fasting is not needed for the Dengue NS1 test.

    Best Time to Get Tested

    The Dengue Antigen test can be conducted at any time during the day.

    Who Should Consider a Dengue NS1 ELISA Test

    You should consider this test if you have recently been in a dengue-endemic area and are experiencing symptoms such as high fever, rash, pain behind the eyes, nausea, vomiting, or muscle and joint pain.

    Cautions Before Taking Dengue NS1 ELISA Test

    No special precautions are required before taking the Dengue NS1 test.

    Why Take a Dengue NS1 Antigen ELISA Test?

    It is advisable to take a Dengue NS1 test if you live in or have visited a region where dengue fever is prevalent and are currently experiencing symptoms of the illness. Dengue fever symptoms typically emerge within 4 to 10 days following a mosquito bite and persist for 3 to 7 days. These symptoms often include a rapid onset of fever up to 104°F or more, along with any of the following:

    • Rash

    • Headache

    • Nausea and vomiting

    • Severe pain behind the eyes

    • Muscle, bone, or joint pain

    When neglected, some individuals might develop dengue haemorrhagic fever, a life-threatening condition that causes abdominal pain, frequent vomiting, nosebleeds, bleeding gums, vomiting blood, blood in stools, and extreme tiredness. This underscores the need for early detection and treatment of dengue.

    The NS1 antigen is present in the blood during the acute phase of infection, making the NS1 test effective within the first 7 days of symptoms. Beyond the first week, the test’s sensitivity decreases.

    The Dengue Antigen test thus helps doctors detect dengue early, confirm the infection, and differentiate it from other similar diseases.

    Dengue NS1 Antigen ELISA Test Results & Interpretation

    The table given below explains the interpretation of the results of a Dengue NS1 ELISA test:

    NS1 ResultsIndex ValuesInterpretation
    Negative≤0.8Retest if NS1 antigen is not found. Further assessment is needed if symptoms persist.
    Equivocal0.8 to 1.1Should undergo testing using an alternative method.
    Positive≥1.1Indicates a dengue virus infection.

    Interference may occur in some cases where the blood sample contains antibodies against test components, such as during dengue virus testing, where cross-reactivity with other Flaviviruses or non-infectious conditions might result in false-positive results or misinterpretation. Thus, it's essential to consider the patient's clinical history for accurate diagnosis. Using both NS1 detection and the IgM Antibody test can enhance diagnostic accuracy for dengue infection. The dengue NS1 ELISA is preferred within the initial 7 days of symptom onset. After 7-10 days, IgG and IgM antibody tests are advised.

    FAQs on Dengue NS1 Antigen ELISA Test in Hyderabad

    The Dengue NS1 Antigen ELISA test price is ₹600. This price includes rapid home sample collection, completed within 60 minutes of scheduling, with results provided in 6 hours.

    The Dengue NS1 Antigen test is performed to detect dengue infection during the initial 7 days of symptom onset.

    No, fasting is not recommended for this test.

    Yes, Orange Health Labs offers a home sample collection for the Dengue NS1 ELISA test. An eMedic will arrive at your location for sample collection within 60 minutes of booking the test (based on slot availability) or at your chosen date and time.

    Follow these steps to book any blood test or health checkup effortlessly on our platform:

    • Search for the Test : Find the Dengue fever NS1 Antigen test in Hyderabad or at home, then choose Orange Health’s page.

    • Confirm Your Booking : Choose the test, check the prerequisites, input your address details, and confirm the booking by selecting a suitable time slot for sample collection.

    • Sample Collection : Our skilled eMedic will collect the sample from your home during the chosen time slot.

    • Lab Processing : The collected sample will be examined at our NABL accredited and ICMR approved labs.

    • Receive Your Reports : Your reports will be sent to you via email, WhatsApp, or our app within 6 hours of sample collection.

    The result of the Dengue NS1 ELISA test is interpreted in this way:

    • Negative: Index values ≤0.8. If NS1 antigen is absent, a retest is recommended.

    • Equivocal: Index values between 0.8 and 1.1. Further testing using an alternative method is suggested.

    • Positive: Index values ≥1.1, which confirms a dengue virus infection.

    The Orange Health Labs eMedic will reach your location within 60 minutes of scheduling your test. The sample is usually taken in under 5 minutes, and you can expect to receive your results online in 6 hours.

    The frequency of testing is based on your doctor's recommendation, which depends on your symptoms, test findings, and any underlying health conditions. Generally, this test is suggested just once, early in the course of dengue infection.

    Within the Dengue NS1 Antigen test, the key parameter is the detection of the NS1 protein, released into the bloodstream during infection.

    The Dengue NS1, IgM, and IgG tests are designed to detect different elements of the virus and the immune response to dengue infection:

    • NS1 test: Detects the NS1 protein, present in the blood during the early phase of dengue infection, detectable from day 1 to day 7 of symptom onset.

    • IgM test: Detects IgM antibodies, which become evident from day 3 to day 5 of infection and last for 2 to 3 months.

    • IgG test: Detects IgG antibodies, which generally appear by day 14 of infection and last for life.

    The combined results of these antigen and antibody tests help confirm a current dengue virus infection.

    Yes, NS1 can persist in the bloodstream of some individuals for a few more days after the acute phase of infection.

    Although a positive NS1 test indicates a dengue infection, it cannot identify the virus's serotype. To determine the serotype, samples must undergo nucleic acid amplification tests (NAAT) like real-time reverse transcriptase-polymerase chain reaction (RT-PCR), which detect and identify the dengue virus's genetic material.

    A negative NS1 test result after exposure to the virus does not rule out dengue infection. Your doctor might recommend additional tests, such as NAAT (RT-PCR) or an IgM antibody test, to verify the diagnosis.

    Without having to visit a physical lab, Orange Health provides the fastest Dengue Fever NS1 Antigen test services near you across Hyderabad at the convenience of your home. Localities include but not limited to Banjara Hills, Jubilee Hills, Gachibowli, Madhapur, Hitech City, Kondapur, Begumpet, Somajiguda, Ameerpet, Panjagutta, Kukatpally, Miyapur, Manikonda, Secunderabad, Tarnaka, Abids, Koti, Mehdipatnam, Dilsukhnagar, Uppal, LB Nagar, Nallagandla, Nizampet, Attapur, Malkajgiri.

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