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    What is a Dengue NS1 Antigen ELISA Test?

    The Dengue NS1 Antigen ELISA test identifies the non-structural protein 1 (NS1) of the dengue virus in blood samples. This antigen, representing any foreign material like viruses entering the body, can be detected during the first 7 days of dengue fever, known as the acute phase. Early detection through the NS1 test allows for timely diagnosis and medical intervention.

    Dengue fever, a mosquito-borne illness, is triggered by one of the four serotypes of the dengue virus from the genus Flavivirus. Upon being bitten by an infected mosquito, the virus enters the bloodstream and starts replicating. This viral activity and the body's immune response cause dengue fever symptoms. The Dengue NS1 test can detect the virus in blood within the first 7 days of symptom onset. Research indicates that NS1 antigen levels in the blood correlate with disease severity, with higher levels found in severe cases. Therefore, the Dengue Antigen test offers valuable diagnostic insight into dengue infections.

    If you live in Mumbai and exhibit symptoms of dengue fever, scheduling a Dengue Antigen test can be crucial. Book your Dengue Antigen test in Mumbai today to determine your dengue status.

    Dengue NS1 Antigen ELISA Test Parameters

    Dengue is transmitted through the bite of Aedes mosquitoes. When a mosquito bites a person with dengue fever and then bites a healthy individual, it spreads the infection. The dengue virus can damage blood components responsible for clotting and maintaining blood vessel structure. This damage, along with certain immune system chemicals, can cause blood to leak from vessels. This may lead to internal bleeding. Therefore, prompt testing is crucial if symptoms arise.

    The Dengue NS1 Antigen test targets a single key parameter: the detection of the dengue NS1 protein, which is released into the blood during infection. This test is performed on a blood sample using special antibodies designed to detect the NS1 antigen. The immune system produces antibodies, which are proteins, in reaction to antigens such as NS1. The identification of NS1 in the blood confirms an acute dengue virus infection, but its absence does not rule out infection. Additional tests may be necessary to confirm the diagnosis.

    Dengue NS1 Antigen ELISA Test Preparation

    Here are key factors to consider before a Dengue Fever NS1 Antigen test:


    No fasting is required for the Dengue NS1 test.

    Best Time to Get Tested

    The Dengue Antigen test can be performed at any time of day.

    Who Should Consider a Dengue NS1 ELISA Test

    This test is recommended if you live in or have travelled to an area with a high prevalence of dengue and exhibit recent symptoms such as high fever, rash, muscle and joint pain, pain behind the eyes, nausea, and vomiting.

    Cautions Before Taking Dengue NS1 ELISA Test

    No special precautions are necessary before taking the Dengue NS1 test.

    Why Take a Dengue NS1 Antigen ELISA Test?

    If you reside in or have travelled to a dengue-prone area and have recently exhibited symptoms of dengue fever, you should take a Dengue NS1 test. Dengue fever symptoms generally appear within 4 to 10 days after a mosquito bite and last for 3 to 7 days. Common symptoms include a sudden high fever of up to 104°F or higher, accompanied by any of the following symptoms:

    • Rash

    • Nausea and vomiting

    • Severe pain behind the eyes

    • Headache

    • Muscle, bone, or joint pain

    If left untreated, some individuals may develop a severe form of dengue called dengue haemorrhagic fever, which can cause bleeding gums, nosebleeds, abdominal pain, frequent vomiting, vomiting blood, blood in stools, and extreme fatigue. The risk of severe dengue highlights the importance of early detection and treatment.

    The NS1 antigen is identifiable in the blood during the acute phase of infection, so the NS1 test is performed within the first 7 days of symptom onset. After this period, the test may lose sensitivity in diagnosing dengue.

    The Dengue Antigen test therefore aids doctors in early dengue detection, confirming infection, and ruling out other illnesses with similar symptoms.

    Dengue NS1 Antigen ELISA Test Results & Interpretation

    The interpretation of Dengue NS1 ELISA test findings is explained in the table below:

    NS1 ResultsIndex ValuesInterpretation
    Negative≤0.8Retest if NS1 antigen is not found. Further assessment is needed if symptoms persist.
    Equivocal0.8 to 1.1Should undergo testing using an alternative method.
    Positive≥1.1Indicates a dengue virus infection.

    It is important to note that interference may occur in some cases where the blood sample contains antibodies against test components. For example, during dengue virus testing, cross-reactivity with other Flaviviruses or non-infectious conditions might lead to false-positive results or misinterpretation. Therefore, considering the patient's clinical history is crucial for an accurate diagnosis. Combining NS1 detection with the IgM Antibody test can improve the accuracy of diagnosing dengue infection. The dengue NS1 ELISA is the preferred test within the first 7 days of symptom onset. After 7-10 days, IgG and IgM antibody tests are recommended.

    FAQs on Dengue NS1 Antigen ELISA Test in Mumbai

    The Dengue NS1 Antigen ELISA test price is ₹600. The price covers the fastest home sample collection, done within 60 minutes of booking. Test results are available in just 6 hours.

    The Dengue NS1 Antigen test is utilised to diagnose dengue infection within the first 7 days after symptoms appear.

    No, you do not need to fast prior to this test.

    Yes, Orange Health Labs offers home sample collection for the Dengue NS1 ELISA test. An eMedic will arrive at your location for sample collection within 60 minutes of booking, depending on slot availability. Alternatively, you can also book the test at your chosen date and time.

    Here is how you can book any blood test or health checkup on our platform:

    • Search for the Test : Look for the Dengue fever NS1 Antigen test in Mumbai or at home, then click on Orange Health’s listing.

    • Review and Confirm : Select the test, review the prerequisites, enter your address, and confirm the booking by choosing a convenient time slot for sample collection.

    • Sample Collection : Our skilled eMedic will visit your home during the selected time slot to collect the sample.

    • Lab Analysis : The collected sample will undergo analysis at our NABL accredited and ICMR approved labs.

    • Receive Your Reports : Expect your reports to be delivered via email, WhatsApp, or our app within 6 hours of sample collection.

    The interpretation of Dengue NS1 ELISA test results is as follows:

    • Negative: Index values ≤0.8. If NS1 antigen is not detected, retesting is recommended.

    • Equivocal: Index values between 0.8 and 1.1. Additional testing with an alternative method is advised.

    • Positive: Index values ≥1.1, indicating a dengue virus infection.

    The Orange Health Labs eMedic will arrive at the location within 60 minutes of booking your test. The sample is generally taken in less than 5 minutes, and you can anticipate receiving your results online in 6 hours.

    Your doctor will recommend the frequency of testing, considering your symptoms, test results, and any underlying conditions. Usually, this test is suggested only once, during the initial stages of dengue infection.

    The Dengue NS1 Antigen test revolves around the singular parameter of detecting the NS1 protein, which is found in the bloodstream during the infection.

    The Dengue NS1, IgM, and IgG tests each identify different components of the virus and the immune response to a dengue infection:

    • NS1 test: Identifies the NS1 protein of the virus, which is present in the blood during the early stages of dengue infection, detectable from days 1 to 7 after symptoms begin.

    • IgM test: Identifies IgM antibodies, which become detectable from days 3 to 5 of dengue infection and remain for 2 to 3 months.

    • IgG test: Identifies IgG antibodies, which typically appear by day 14 of dengue infection and remain for life.

    The results of these antigen and antibody tests are combined to confirm a current dengue virus infection.

    Yes, it is possible for NS1 to be detected in the blood of some individuals for several days after the acute phase of infection has passed.

    While a positive NS1 test result confirms a dengue infection, it does not determine the virus's serotype. For serotype identification, nucleic acid amplification tests (NAAT), such as real-time reverse transcriptase-polymerase chain reaction (RT-PCR), are used to detect the virus's genetic material and identify its serotype.

    If you have been exposed to the virus but receive a negative NS1 test result, it does not eliminate the possibility of a dengue infection. Your doctor may suggest further tests such as NAAT (RT-PCR) or an IgM antibody test to confirm the diagnosis.

    Orange Health offers the quickest Dengue Fever NS1 Antigen test services near you in Mumbai from the comfort of your own home, without the need to attend a physical lab. Localities include but are not limited to Colaba, Nariman Point, Marine Drive, Malabar Hill, Churchgate, Worli, Dadar, Parel, Byculla, Bandra, Andheri, Juhu, Ghatkopar, Powai, Chembur, Vashi, Belapur, Santacruz, Khar, Versova, Lokhandwala, Goregaon, Borivali, Kandivali, Mulund, Bhandup, Kurla.

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