What are the limitations of the dengue test?

The Dengue ELISA test has several limitations. The accuracy of the test can be affected by the timing of the test, with early or late testing potentially yielding false-negative results due to undetectable antibody levels. Cross-reactivity with other flaviviruses such as Zika, West Nile, and yellow fever can lead to false-positive results. The test may also have reduced sensitivity in individuals with weakened immune systems who produce lower levels of antibodies. Additionally, the ELISA test requires specialised equipment and trained personnel, making it less accessible in resource-limited settings. Confirmatory testing with methods such as PCR or repeat ELISA testing may be needed to overcome these limitations and ensure accurate diagnosis.