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    What is a Dengue NS1 Antigen ELISA Test?

    The Dengue NS1 Antigen ELISA test is designed to detect the non-structural protein 1 (NS1) of the dengue virus in a blood sample. Any foreign material that enters the body (such as viruses) is called an antigen. Detection of NS1 is possible during the acute phase (or first 7 days) of dengue fever. This signifies that the NS1 test can help in the early diagnosis of dengue and the initiation of appropriate medical care.

    Dengue fever, a mosquito-borne illness, is caused by one of the four serotypes (or strains) of the dengue virus that belongs to the genus Flavivirus. When an infected mosquito bites a person, the dengue virus enters the bloodstream and begins to replicate. The virus and the immune system response result in the symptoms associated with dengue fever. The Dengue NS1 test can detect the virus in the blood within the first 7 days of experiencing symptoms. Studies also show that the levels of NS1 antigen in the blood correlate with the severity of the disease, with higher levels seen in those with severe infection. Thus, Dengue antigen test can provide valuable diagnostic information in dengue infection.

    Dengue NS1 Antigen ELISA Test Parameters

    Dengue is spread by the bite of Aedes mosquitoes. When a mosquito bites a person with dengue fever and then bites a healthy person, it transmits the infection to the latter. The dengue virus can damage components of your blood that are responsible for forming clots and maintaining the structure of your blood vessels. This damage, together with certain chemicals produced by your immune system, can cause blood to leak from your vessels and result in internal bleeding. Therefore, it is important to get tested promptly if symptoms appear.

    The Dengue NS1 Antigen test focuses on just one key parameter – the detection of dengue NS1 protein which is released into the blood during the infection. The NS1 test is performed on a blood sample and typically uses special antibodies designed to detect the presence of the NS1 antigen. Antibodies are proteins produced by our immune system in response to antigens, like NS1. The presence of NS1 in the blood confirms an acute dengue virus infection. However, the absence of NS1 in a sample does not rule out infection. Additional tests may be ordered by your doctor to validate the diagnosis.

    Dengue NS1 Antigen ELISA Test Preparation

    Given below is an overview of the test's preparation:


    The Dengue NS1 test does not need fasting.

    Best Time to Get Tested

    The Dengue NS1 Antigen test can be done at any time of the day.

    Eligibility for Dengue NS1 ELISA Test

    Dengue NS1 test should be done if you live in or have travelled to an area where dengue is common and have been having recent symptoms indicative of dengue, such as high fever, rash, muscle and joint pain, pain behind the eyes, nausea and vomiting.

    Cautions Before Taking Dengue NS1 ELISA Test

    No special cautions are needed before taking a Dengue NS1 test.

    Why Take a Dengue NS1 Antigen ELISA Test?

    You should take a Dengue NS1 test if you live in or have been to an area where dengue fever is common and have recently been showing symptoms of dengue. The symptoms of dengue fever usually arise within 4 to 10 days after a mosquito bite and continue for 3 to 7 days. Symptoms typically include a sudden fever reaching up to 104°F or above along with any of the following symptoms:

    • Rash

    • Nausea and vomiting

    • Severe pain behind the eyes

    • Headache

    • Muscle, bone or joint pain

    If neglected, some individuals may develop a life-threatening worsening of symptoms called dengue hemorrhagic fever that causes nose bleeds, bleeding gums, abdominal pain, frequent vomiting, throwing up blood, blood in stools, and extreme tiredness. The risk of severe dengue underscores the need for early detection and treatment of this infection.

    Since the NS1 antigen is detectable in the blood during the acute phase of infection, the NS1 test is done in the first 7 days of experiencing symptoms. After the first week, the NS1 test may not be as sensitive in diagnosing dengue.

    The Dengue Antigen test thus helps healthcare providers with early detection of dengue, confirming dengue infection, and ruling out other non-dengue illnesses that have similar symptoms.

    Dengue NS1 Antigen ELISA Test Results & Interpretation

    The below table explains the interpretation of results of a Dengue NS1 ELISA test:

    NS1 ResultsIndex ValuesInterpretation
    Negative≤0.8Retest if NS1 antigen isn't found. Further assessment is needed if symptoms persist.
    Equivocal0.8 to 1.1Should undergo testing using an alternative method.
    Positive≥1.1Indicates a dengue virus infection.

    It is important to note that interference may occur in some cases where the blood sample contains antibodies against test components. For example, when testing for dengue virus, the test might also detect antibodies from cross-reactivity with other Flaviviruses or other non-infectious conditions, potentially leading to false-positive results or misinterpretation of the test. Therefore, when interpreting test results, it's essential to consider the patient's clinical history to ensure an accurate diagnosis. In such cases, using both NS1 detection and IgM Antibody test can enhance the accuracy of diagnosing dengue infection. It’s also important to note that the dengue NS1 ELISA is the preferred test within the initial 7 days of symptom onset. After 7-10 days, dengue fever IgG and IgM antibody tests are recommended.

    FAQs on Dengue NS1 Antigen ELISA Test in Bangalore

    The Dengue NS1 Antigen ELISA test price is ₹ 250 . This price includes the fastest home sample collection within 60 of booking, and the reports are available in 6 hours.

    The Dengue NS1 Antigen test is used to diagnose dengue infection in the initial 7 days after symptom onset.

    No, fasting is not needed for this test.

    Orange Health Labs provides free home sample collection services. An eMedic will arrive at your location for sample collection within 60 minutes of booking the test or at your chosen date and time.

    Booking a Dengue fever NS1 Antigen test in Bangalore is easy. Simply follow these steps:

    • Search for “Dengue NS1 Antigen test near me” on your browser and select Orange Health Labs. You may also visit the Orange Health app or website.

    • Select the option “book Dengue NS1 Antigen test at home in Bangalore” and enter your details.

    • Choose your preferred date, time, and location for blood sample collection.

    • An experienced eMedic will arrive at your location within an hour of booking or at your scheduled time to collect the blood sample.

    • Your sample will be sent to our NABL- and ICMR-certified lab for testing.

    • Test results will be delivered to you online within 6 hours via email, WhatsApp, or on the app if you’re in Bangalore.

    The Dengue NS1 ELISA test results are interpreted as follows:

    • Negative: Index values ≤0.8. If NS1 antigen isn't found, retesting is recommended.

    • Equivocal: Index values between 0.8 and 1.1. Further testing with an alternative method is suggested.

    • Positive: Index values ≥1.1, indicating a dengue virus infection.

    The Orange Health Labs eMedic will reach your location within 60 of booking your test. The sample is usually taken in less than 5 minutes, and you can expect to receive your results online in 6 hours.

    The frequency of testing depends on your doctor's recommendation. 5 This generally depends on your symptoms, test results, and any underlying conditions. Usually, this test is recommended only once, in the early stages of dengue infection.

    The sole parameter of the Dengue NS1 Antigen test is detecting the NS1 protein, which is released into the bloodstream during the infection.

    The Dengue NS1, IgM, and IgG tests detect different components of the virus and the immune response to dengue infection:

    • NS1 test: Detects the NS1 protein of the virus which is present in the blood during the early phase of dengue infection. NS1 can be detected from days 1 to 7 of dengue symptom onset.

    • IgM test: Detects IgM antibodies, which become detectable from day 3 to day 5 of dengue infection and persists for 2 to 3 months.

    • IgG test: Detects IgG antibodies, which generally appear by day 14 of dengue infection and persists for life.

    The results of the antigen and antibody tests are correlated to confirm a current dengue virus infection.

    Yes, in some individuals, NS1 can be detected in the blood for a few more days after the acute phase of infection.

    Although a positive NS1 test result indicates a dengue infection, it does not identify the serotype of the virus. If serotype identification is needed, the sample is tested using nucleic acid amplification tests (NAAT), such as real-time reverse transcriptase-polymerase chain reaction (RT-PCR). These tests detect the genetic material of the dengue virus and identify its serotype.

    If you have been exposed to the virus but have a negative NS1 test result, it doesn't rule out a dengue infection. Your doctor may recommend additional tests such as NAAT (RT-PCR) or IgM antibody test to validate the diagnosis.

    Without having to visit a physical lab, Orange Health provides the fastest Dengue Fever NS1 Antigen test services near you across Bangalore at the convenience of your home. Localities include but not limited to Adugodi, Banashankari, Bannerghatta, Basavanagudi, Bellandur, BTM Layout, Chamrajpet, Cottonpet, CV Raman Nagar, Domlur, Electronic City, Frazer Town, Giri Nagar, Hebbal, Hennur, Hosur, HSR Layout, Jakkur, Jalahalli, Jaya Nagar, Kadugodi, Kammanahalli, Kengeri, Koramangala, Mahalakshmi Layout, Madiwala, Magadi Road, Malleshwaram, Marathahalli, Mathikere, Mysore Road, Nagarbhavi, Nandhini Layout, Old Airport Road, Peenya, RT Nagar, Rajaji Nagar, Rajarajeshwari Nagar, Sadashivnagar, Sahakara Nagar, Sanjay Nagar, Sarjapur, Seshadripuram, Shanthi Nagar, Shivaji Nagar, Sri Nagar, Tyagarajnagar, Uttarahalli, Vasanth Nagar, Vidyaranyapura, Vijaya Nagar, Whitefield, Yelahanka, Yeshwantpur.

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