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    What is the Anti Nuclear Antibody (ANA) (IFA) Test?

    The ANA test, or Anti-nuclear Antibody test, measures the presence and patterns of antinuclear antibodies in the blood. Normally, these antibodies help the immune system fight off harmful invaders like bacteria and viruses. However, in autoimmune diseases, the immune system can mistakenly attack healthy cells. Antinuclear antibodies target the nucleus, the cell's core, hence the name.

    This test aids doctors in diagnosing autoimmune diseases like systemic lupus erythematosus (SLE) or rheumatoid arthritis by analysing ANA levels along with other tests, symptoms, and physical examination findings. The combined information allows doctors to confirm if the immune system is attacking the body and to determine the appropriate treatment or management plan.

    In Delhi, if you are experiencing symptoms such as joint pain, persistent fatigue, or recurring rashes, getting an ANA test could help detect autoimmune disorders early, allowing for timely treatment initiation.

    Anti Nuclear Antibody (ANA) (IFA) Test Parameters

    The ANA IFA test evaluates two key aspects: the titre value and the fluorescent pattern.

    Titre Value: This measures the concentration of antinuclear antibodies (ANAs) in your blood. To determine this, the blood sample is systematically diluted until the antibodies are no longer visible. The highest dilution level where antibodies remain detectable is noted as the titre. A higher titre indicates a greater quantity of antibodies and a higher likelihood of an autoimmune disorder. Pattern: The test also examines how these antibodies bind to various parts of the cell using a special fluorescent dye, which glows under a microscope. This glowing pattern helps doctors identify specific autoimmune diseases like lupus, rheumatoid arthritis, or Sjögren’s syndrome. By analyzing these patterns, doctors can decide if further testing is needed to determine the presence of specific antibodies.

    The ANA test employs the Indirect Immunofluorescence (IFA) technique, which helps visualize the distinct patterns of these antibodies and provides critical insights for diagnosing autoimmune conditions.

    Anti Nuclear Antibody (ANA) (IFA) Test Preparation

    Before arranging an ANA test at home, consider these important guidelines:

    • Prerequisites

    Fasting is not required for the ANA test.

    • Best Time to Get Tested

    You can have the ANA test at any time of the day.

    • Who Should Get an ANA Profile Test?

    An ANA test may be recommended if your doctor suspects an autoimmune disorder. It is particularly relevant if you are experiencing symptoms such as fever, skin changes or rashes, severe fatigue, joint pain, stiffness, swelling, or muscle aches. This test helps detect specific antibodies in the blood, aiding in the diagnosis of autoimmune conditions.

    • Cautions Before Taking the ANA Profile Test

    No special precautions or preparations are usually needed for the ANA test.

    Why Take the Anti Nuclear Antibody (ANA) (IFA) Test?

    The ANA test is used to detect autoimmune disorders by identifying specific antibodies in your blood. It helps diagnose a variety of conditions, including:

    • Rheumatoid Arthritis: Mainly affects the joints, leading to pain and swelling.

    • Systemic Lupus Erythematosus (SLE): Impacts multiple body parts, such as the skin, joints, and organs.

    • Sjögren's Syndrome: Affects glands responsible for producing tears and saliva.

    • Scleroderma: A rare condition that involves the skin and internal organs.

    • Autoimmune Hepatitis: Causes liver inflammation.

    • Addison Disease: Affects adrenal glands, resulting in symptoms like fatigue and weakness.

    Doctors may recommend the ANA IFA test if you experience symptoms like fever, joint pain, rashes, severe fatigue, or muscle aches, to help determine if an autoimmune disorder is the cause.

    Anti Nuclear Antibody (ANA) (IFA) Test Results & Interpretation

    The ANA test checks for antibodies that target the nuclei of your cells, which can help identify autoimmune diseases.

    • Negative Result: No antibodies were found, indicating a lower likelihood of an autoimmune disorder, though it doesn’t completely rule one out.

    • Positive Result: The presence of these antibodies may suggest an autoimmune disease, a viral infection, or other health issues.

    • Weakly Positive Result: If results are weakly positive, it's recommended to repeat the test in 6-8 weeks for further assessment.

    If your ANA test is positive, specific antibody patterns can help pinpoint the type of autoimmune condition:

    • Homogeneous Pattern: Commonly seen in lupus, indicating antibodies against DNA and histones.

    • Dense Fine Speckled (DFS): Can occur in healthy individuals and those with inflammatory conditions.

    • Speckled Pattern: May suggest lupus, Sjögren's syndrome, or rheumatoid arthritis, indicating antibodies against various nuclear proteins.

    • Centromere Pattern: Associated with certain types of sclerodermas, targeting cell division-related proteins.

    • Nuclear Membrane Pattern: Connected to chronic fatigue syndrome and collagen-related diseases.

    • Nuclear Dot Pattern: Often linked to liver disease and rheumatic conditions, showing antibodies against specific nuclear proteins.

    • Nucleolus Pattern: Suggestive of muscle and skin disorders.

    • Cytoplasmic Patterns: May indicate liver disease, Sjögren's syndrome, and muscle disorders.

    A positive ANA test alone does not diagnose an autoimmune disease. Additional tests and clinical evaluation are necessary to determine the exact condition.

    FAQs on Anti Nuclear Antibody (ANA) (IFA) Test in Delhi

    The cost of the ANA test is ₹ 1300 in Delhi. This price includes home sample collection within 60 minutes after booking, with reports available in 111 hours.

    Yes, Orange Health Labs provides home sample collection services for the ANA test in Delhi. The sample can be collected at your convenience within 60 minutes after booking, subject to availability.

    The ANA test is used to detect antibodies that target the cell's nucleus, helping diagnose autoimmune diseases like lupus and rheumatoid arthritis. A positive result may indicate an autoimmune condition.

    No, fasting is not necessary for the ANA blood test.

    To book an ANA test or any health checkup with Orange Health Labs, follow these steps:

    • Search for the Test: Look for the Antinuclear Antibody/ANA test available in Delhi or at home on our platform and navigate to Orange Health’s test page.

    • Review and Confirm: Choose the test, review any prerequisites, input your location, and finalize your booking by selecting a convenient time for sample collection.

    • Sample Collection: At the scheduled time, a trained eMedic will visit your home to collect the sample.

    • Laboratory Analysis: The sample will be sent to our ICMR-approved and NABL-accredited lab for processing.

    • Receive Your Results: Your test results will be delivered via email or WhatsApp within 111 hours, and can also be accessed through our app.

    ANA test results are interpreted as follows: A negative result suggests a low likelihood of having an autoimmune disease, while a positive result indicates the presence of antibodies, which could point to an autoimmune condition. Higher antibody levels generally signal more severe disease activity, and further testing may be necessary for a comprehensive diagnosis.

    After booking your ANA test, Orange Health Labs will collect your sample within approximately 60 minutes. The actual collection takes just a few minutes, and you can expect your results to be available online within 111 hours.

    The frequency of ANA testing will depend on your medical condition and should be determined by your doctor.

    The ANA test checks for the presence and patterns of antibodies in your blood. A low titre suggests a lower likelihood of autoimmune disease, while a high titre may indicate a more active disease. The specific patterns, such as homogeneous or speckled, help diagnose particular autoimmune conditions like lupus or rheumatoid arthritis.

    Orange Health provides ANA testing throughout Delhi, offering the convenience of home collection. Our services cover numerous localities across the city including Connaught Place, Chandni Chowk, Karol Bagh, Lajpat Nagar, South Extension, Greater Kailash, Hauz Khas, Saket, Vasant Kunj, Dwarka, Rohini, Pitampura, Janakpuri, Rajouri Garden, Mayur Vihar, Preet Vihar, Shahdara, Laxmi Nagar, Green Park, Safdarjung Enclave, Defence Colony, New Friends Colony, Kalkaji, Malviya Nagar, Nehru Place.

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    References

    1. Medline Plus. ANA (Antinuclear Antibody) Test: MedlinePlus Medical Test [Internet]  Available from:  https://medlineplus.gov/lab-tests/ana-antinuclear-antibody-test/
    2. Antinuclear Antibodies (ANA) Test [Internet].  Available from:  https://www.columbiadoctors.org/health-library/test/antinuclear-antibodies-ana-test/
    3. Fastest Anti-nuclear Antibody (ANA) (IFA) Test in Delhi at Best Price + Reports in 6 Hours [Internet].  Available from:  https://www.orangehealth.in/lab-test-bangalore/anti-nuclear-antibody?utm_source=seo_search&utm_medium=google&utm_campaign=seo&utm_term=anti-nuclear-antibody