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    What Is the HLA-B27, Qualitative PCR Test?

    HLA B27 Qualitative PCR Test is a blood test used to check whether the white blood cells in your blood have the HLA-B27 protein, which is component of the immune system. This protein aids the body's defence against infections, although in some cases, the presence HLA-B27 is linked to an increased risk of autoimmune disorders. Autoimmune diseases occur when the immune system targets healthy tissues because it considers them as dangers for the body.

    HLA-B27 is frequently associated with the following conditions:

    • Ankylosing Spondylitis: A painful and stiffening form of arthritis affecting the spine.

    • Juvenile arthritis: Children's inflammatory joint disease.

    • Reactive arthritis: Inflammation of the joints brought on by an infection is known as

    The presence of HLA-B27 in the blood as shown by a positive test result is an indicator of an increased risk of developing certain autoimmune diseases, although it is not confirmative. Many people with HLA-B27 may never develop these conditions. For further confirmation, additional tests such as the Erythrocyte Sedimentation Rate (ESR), C-reactive protein (CRP), X-ray, MRI scan, and urine test may be required.

    The absence of HLA-B27 in test results suggests a lower likelihood of developing these conditions. For individuals needing organ or tissue transplants, mismatched HLA types can lead to transplant rejection.

    If you are located in Delhi, getting the HLA B27 Qualitative PCR test at home can be convenient with the availability of home sample collection and timely results.

    HLA-B27, Qualitative PCR Test Parameters

    The HLA B27 Qualitative PCR Test assess one parameter: the presence of the HLA-B27 antigen on white blood cells in blood.

    The human leukocyte antigen (HLA) protein is a component of the immune system that helps recognise the body's own cells and distinguish them from foreign invaders like viruses and bacteria. The HLA-B27 antigen is inherited from parents and its presence or absence is determined by genetics. You have a higher chance of getting ankylosing spondylitis or any other disease linked to HLA-B27 if you have it yourself and a close relative has it.

    HLA proteins are classified into two groups: Class I (A, B, C) and Class II (DR, DP, DQ). The HLA proteins vary from one individual to other and play a crucial role in immune mediation.

    HLA-B27 has about 15 distinct subtypes, the most prevalent of which are HLA-B2705 and HLA-B2702. The likelihood of acquiring autoimmune illnesses may be influenced by the existence of specific subtypes. It is currently unclear how HLA-B27 subtypes and disease risk are related, and possessing HLA-B27 does not guarantee that a person will get sick. Nonetheless, its existence may offer valuable insights into the diagnosis of specific autoimmune conditions.

    HLA-B27, Qualitative PCR Test Preparation

    These points must be kept in mind if you are planning to take the HLA B27 Qualitative PCR test at home:

    • Prerequisites : There is no need for any special preparations, like fasting, before taking the HLA B27 Qualitative PCR test.

    • Best Time to Get Tested : The test can be done at any time of the day based on convenience.

    • Who Should Get a HLA B27 Qualitative PCR Test? : The HLA B27 Qualitative PCR test is advised for people who have suspected autoimmune diseases, recurrent eye inflammation (uveitis), persistent joint pain, stiffness, or swelling, or to test computability in individuals who need organ or tissue transplants.

    • Cautions Before Taking a HLA B27 Qualitative PCR Test : There are no specific precautionary measures to be taken before the HLA B27 Qualitative PCR test.

    Why Take the HLA-B27, Qualitative PCR Test?

    The HLA-B27 test may be recommended in various medical situations where autoimmune disorders or inflammatory conditions are suspected. It could be advised if you have:

    • Ongoing joint pain, stiffness, or swelling, which may point to an autoimmune disorder.

    • Chronic inflammation affecting the spine, neck, chest, eyes, or joints, suggesting conditions like ankylosing spondylitis or reactive arthritis.

    • Frequent uveitis with no obvious cause, potentially related to an autoimmune condition.

    This test is also used:

    • To screen for ankylosing spondylitis or other autoimmune diseases associated with HLA-B27 to evaluate the risk of developing these conditions.

    • Before an organ or bone marrow transplant to assess compatibility between the donor and recipient.

    The HLA-B27 Qualitative PCR test is often performed alongside other tests, such as CCP antibodies, CRP, ESR, rheumatoid factor, and X-rays, to evaluate inflammation, joint damage, and autoimmune disorders.

    FAQs on HLA-B27, Qualitative PCR Test in Delhi

    The price of HLA B27 Qualitative PCR test in Delhi is ₹2500. This price includes home sample collection done within 60 minutes of booking at Orange Health Labs. The test results are delivered within 37 hours after sample collection.

    Yes, at Orange Health Labs, free home sample collection for the HLA B27 Qualitative PCR test is available. A trained eMedic professional will arrive at your location after the booking, within 60 minutes to collect the sample conveniently.

    The HLA B27 Qualitative PCR test is used to detect the presence of the HLA-B27 antigen, which is linked to autoimmune disorders like ankylosing spondylitis, reactive arthritis, and psoriatic arthritis. It helps determine compatibility for organ transplant.

    No, fasting is not required for the HLA B27 Qualitative PCR test, and the test can be taken at any time of the day.

    To schedule an HLA B27 Qualitative PCR test with Orange Health Labs, simply follow these steps:

    • Locate the Test: Search for "HLA B27 Qualitative PCR test" and choose Orange Health Labs from the available options.

    • Confirm Your Appointment: Provide your details, select your location, and pick a suitable time for sample collection.

    • Sample Collection: A trained eMedic professional will visit your home to collect the blood sample.

    • Testing & Results: The sample will be analysed at an NABL-accredited, ICMR-approved laboratory, and your results will be sent via email or WhatsApp within 37 hours of sample collection.

    The HLA-B27 Qualitative PCR test shows the result as positive or negative. A negative result is normal, while a positive result indicates a higher likelihood for developing autoimmune diseases, although it doesn't confirm them. 1,3

    Test results are generally available within 37 hours after the sample is collected.

    The frequency of testing depends on your symptoms, health condition, and your doctor's recommendation.

    The HLA B27 Qualitative PCR test checks for a single parameter: the presence of HLA-B27 antigen in the blood.

    The HLA B27 Qualitative PCR test is available in multiple locations across Delhi, including Connaught Place, Chandni Chowk, Karol Bagh, Lajpat Nagar, South Extension, Greater Kailash, Hauz Khas, Saket, Vasant Kunj, Dwarka, Rohini, Pitampura, Janakpuri, Rajouri Garden, Mayur Vihar, Preet Vihar, Shahdara, Laxmi Nagar, Green Park, Safdarjung Enclave, Defence Colony, New Friends Colony, Kalkaji, Malviya Nagar, Nehru Place.

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    References

    1. HLA-B27 antigen: MedlinePlus Medical Encyclopedia.  Available from:  https://medlineplus.gov/ency/article/003551.htm
    2. HLA-B27 Antigen.  Available from:  https://www.urmc.rochester.edu/encyclopedia/content?ContentTypeID=167&ContentID=hla_b27_antigen
    3. Human leukocyte antigen (HLA) B27 | Pathology Tests Explained.  Available from:  https://www.pathologytestsexplained.org.au/ptests-pro.php?q=HLA+B27