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    Widal Test (Slide Method)34225027% off
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    What is a Widal Test (Slide Method) ?

    The Widal test is a diagnostic blood test introduced by F. Widal in 1896 to measure antibody titres in suspected typhoid or paratyphoid fever cases, collectively termed enteric fever. Antibody titres reflect the immune response by indicating the presence and levels of specific antibodies in the blood in response to foreign substances (antigens). The Widal test targets antibodies against the O and H antigens of Salmonella Typhi and the H antigens of Salmonella Paratyphi . It uses a suspension medium with killed Salmonella Typhi bacteria as the antigen to diagnose typhoid fever in patients with fever. Therefore, individuals with symptoms of enteric fever may benefit from undergoing a Widal test.

    Given its role in diagnosing enteric fever, the Widal test can be a critical diagnostic tool aiding in timely detection and treatment. Therefore, if you live in Delhi and are experiencing symptoms of enteric fever, consider getting a Widal test in Delhi today.

    Widal Test (Slide Method) Parameters

    The Widal test, an agglutination test, identifies cell clumping caused by specific antibodies (agglutinins) in the blood reacting to particular antigens. These antibodies target two essential antigens of Salmonella Typhi : the O antigen and the H antigen. The test also incorporates the H antigens of Salmonella Paratyphi . Detecting these antibodies aids in diagnosing enteric fever by indicating the body's immune response to these bacterial infections.

    The Widal test has four parameters, which represent the levels of antibody titres against the bacteria causing enteric fever. The parameters include:

    • Salmonella Typhi O (TO)

    • Salmonella Typhi H (TH)

    • Salmonella Paratyphi A, H (AH)

    • Salmonella Paratyphi B, H (BH)

    Widal Test (Slide Method) Preparation

    Before booking the Widal test in Delhi, consider the following factors:

    Prerequisites

    No fasting is needed for the Widal test.

    Best Time to Get Tested

    You can schedule the Widal test at any time during the day.

    Who Should Get a Widal Test

    People showing symptoms such as fatigue, fever, gastrointestinal issues (abdominal pain, diarrhoea, or constipation), and headache, especially if they have been in areas with an elevated risk of typhoid or paratyphoid fever, should undergo a Widal test.

    Cautions Before Taking the Widal Test

    There are no special precautions required for this test.

    Why Take a Widal Test (Slide Method) ?

    Typhoid bacteria have an incubation period of 6 to 14 days, and in some cases, up to 30 days. This period is the interval between infection and the appearance of symptoms. Paratyphoid bacteria have an incubation period of 1 to 10 days. Symptoms often worsen gradually over a period of 3 to 4 days.

    A doctor might request a Widal test if you exhibit symptoms of typhoid fever. Paratyphoid fever shows similar, but less severe, symptoms. These signs and symptoms include:

    • High fever or chills

    • Headache

    • Loss of appetite

    • Constipation

    • Red rash on the chest and abdomen

    • Extreme fatigue

    • Feeling unwell, weak, or sick

    • Pain in the abdomen

    • Diarrhoea

    • Sweating

    • Blood in the stool

    • Sore throat and persistent cough

    • Bleeding from the nose

    • Weight loss

    • Decreased heart rate

    • Difficulty in concentrating

    • Confusion or hallucinations

    • Enlarged spleen or liver

    • Poisoning of blood

    It is important to keep in mind that typhoid vaccination does not offer complete immunity, with 20–50% of vaccinated people potentially contracting the disease upon exposure.

    Individuals at higher risk for typhoid may be recommended to undergo a Widal test, including:

    • Young children, as they are more susceptible to these diseases than adults.

    • People who are in close contact with an infected individual.

    • Travellers to regions with high rates of typhoid and paratyphoid, such as India, Bangladesh, the Middle East, and Africa.

    Widal Test (Slide Method) Results & Interpretation

    Below is a breakdown of the results of the Widal test:

    TypeAntibodyTitreInterpretation
    Salmonella TyphiTO<1:80Negative
    Salmonella TyphiTH<1:80Negative
    Salmonella ParatyphiAH<1:80Negative
    Salmonella ParatyphiBH<1:80Negative

    Interpretation

    A titre under 1:80 is considered negative, meaning the patient likely does not have an infection.

    Titres of 1:80 or more are usually deemed positive, though the exact threshold can vary by population and region. A rising titre, noted through two or more samples taken 4 to 6 days apart after symptom onset, is more indicative than a single high titre. The results should be correlated with the patient’s clinical findings to make a final diagnosis.

    Limitations

    Elevated antibody titres in vaccinated patients may result in false-positive outcomes. Similarly, results may be misleading for individuals with previous infections or immunisations.

    Agglutinins typically appear in the blood by the end of the first week of infection, so samples taken earlier may show negative results.

    While the serological findings from this test are useful, they should not substitute culture-based methods for identifying the causative organisms. Efforts should be made to recover and identify the pathogens through culture and biochemical tests.

    FAQs on Widal Test (Slide Method) in Delhi

    The Widal price is ₹ 250. This fee includes the fastest home sample collection within 60 minutes of scheduling, and the reports are available within 6 hours.

    A Widal test, or blood test for typhoid, is conducted to detect antibodies in the blood against particular antigens of Salmonella bacteria, primarily Salmonella Typhi and Salmonella Paratyphi . This test aids in diagnosing enteric fever, which includes typhoid and paratyphoid fever, by showing the body's immune response to these bacterial infections.

    No, you do not need to fast for the Widal test or Typhoid test at home.

    Yes, you can schedule a home sample collection for the Widal Blood test or Typhoid Blood test with Orange Health Labs and enjoy the convenience of sample collection within 60 minutes of booking, subject to slot availability.

    To book a blood test or health checkup on our platform, follow these steps:

    Search for the Test : Locate the Widal test in Delhi or the Typhoid test in Delhi and click on Orange Health’s page.

    Review and Book : Choose the test, check for any prerequisites, enter your address, and confirm the booking by selecting a suitable time slot for sample collection.

    Sample Collection : Our trained eMedic will arrive within your chosen time slot to collect the sample at your home.

    Lab Processing : The collected sample will be sent to our NABL accredited and ICMR approved labs for analysis.

    Receive Results : You will receive the reports of your Widal test at home via email or WhatsApp within 6 hours. They will also be accessible on our app.

    The Widal test does not have a defined 'normal' range. Instead, titres below 1:80 are generally regarded as negative, while titres of 1:80 or greater could indicate infection. Nonetheless, interpretation depends on clinical variables and the disease's prevalence within the region.

    Upon sample collection, results will be delivered to you via WhatsApp and the app within 6 hours if you are based in Delhi. Timeframes may differ in other locations.

    The frequency of Widal testing is based on your doctor's guidance. If you have been diagnosed with typhoid or paratyphoid fever, your doctor may suggest having this test done every 2 weeks, tailored to your unique clinical situation and symptoms.

    In the Widal test, the parameters assessed include the levels of antibodies directed against distinct antigens of Salmonella bacteria, which encompass the O and H antigens of Salmonella Typhi and the H antigens of Salmonella Paratyphi .

    The presence of antibodies against Salmonella bacteria, as indicated by the Widal test, supports the diagnosis of typhoid and paratyphoid fever.

    Mixing serum and antigen occurs directly on a slide in the Slide method, while the Tube method mixes them in test tubes.

    Blood tests are generally safe. You might feel some discomfort at the site where the needle is inserted during sample collection, but it typically resolves quickly.

    If the Widal test is performed too early, it may yield a false-negative result, wrongly suggesting no antibodies are present. Conversely, a false-positive result, possibly caused by prior infections or vaccinations, inaccurately suggests the presence of antibodies.

    Without having to visit a physical lab, Orange Health provides the fastest Widal test services near you across Delhi at the convenience of your home. Localities include but are not limited to Connaught Place, Chandni Chowk, Karol Bagh, Lajpat Nagar, South Extension, Greater Kailash, Hauz Khas, Saket, Vasant Kunj, Dwarka, Rohini, Pitampura, Janakpuri, Rajouri Garden, Mayur Vihar, Preet Vihar, Shahdara, Laxmi Nagar, Green Park, Safdarjung Enclave, Defence Colony, New Friends Colony, Kalkaji, Malviya Nagar, Nehru Place.

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